ORIGINAL ARTICLE |
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Year : 2016 | Volume
: 3
| Issue : 1 | Page : 32-40 |
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Histological and biochemical evaluation of the antidiabetic potentials of s-allyl-cysteine and mangiferin in type 2 diabetic rat models
IA Iliya1, B Mohammed2, SA Akuyam3, JD Yaro4, JA Timbuak1, M Tanko1, AJ Nok5
1 Department of Human Anatomy, Faculty of Human Medicine, Ahmadu Bello University, Zaria-Kaduna State, Nigeria 2 Department of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria-Kaduna State, Nigeria 3 Department of Chemical Pathology, Ahmadu Bello University, Zaria-Kaduna State, Nigeria 4 Department of Histopathology, Ahmadu Bello University, Zaria-Kaduna State, Nigeria 5 Department of Biochemistry, Ahmadu Bello University, Zaria-Kaduna State, Nigeria
Correspondence Address:
I A Iliya Department of Human Anatomy, Ahmadu Bello University, Zaria Nigeria
Source of Support: None, Conflict of Interest: None | Check |
DOI: 10.4103/2384-5147.176306
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Biochemical and histological assessments was carried out on the anti-diabetic potentials of s-allyl-cysteine (SAC) and mangiferin (MAN) in Wistar rats models induced with type 2 diabetes mellitus by feeding the rats with a high fat diet for 10 weeks followed by a low dose injection of streptozotocin (40 mg/kg). Therapeutic interventions with 50 mg/kg body weight of (SAC) and 40 mg/kg body weight of (MAN) and a combined therapy (COM) of both SAC and MAN in equal volume ratios (1:1) for 14 days showed that there was a significant improvement in the glucose tolerance ability of the diabetic treated rats (P < 0.05). Consequently, the activities of hepatic pathophysiological enzymes (Alanine aminotransferase, ALT; Aspartate aminotransferase, AST; Alkaline phosphatase, ALP), as well as glycosylated haemoglobin levels were significantly ameliorated in the diabetic treated rat models (P < 0.05). Histomorphometrical examinations of stained pancreatic tissue sections showed a reduction in the total surface area of islets in the diabetic control rats which was however significantly improved in the diabetic treated rats (P < 0.05) except the COM treated group. Subsequent histomorphological evaluation also showed necrosis and vacuolization of islet β-cells to be reasonably reduced in the diabetic treated rats but to a lesser extent in the COM treated group. |
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