• Users Online: 1090
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2016  |  Volume : 3  |  Issue : 1  |  Page : 25-31

Immunohistochemical evaluation of the antidiabetic potentials of S-allyl-cysteine (Garlic) and mangiferin (Mango) in type 2 diabetic rat models


1 Department of Human Anatomy, Faculty of Human Medicine, Ahmadu Bello University, Zaria-Kaduna State, Nigeria
2 Department of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria-Kaduna State, Nigeria
3 Department of Chemical Pathology, Ahmadu Bello University, Zaria-Kaduna State, Nigeria
4 Department of Histopathology, Ahmadu Bello University, Zaria-Kaduna State, Nigeria
5 Department of Integrated Science (Biology), College of Education, Minna, Niger State, Nigeria

Correspondence Address:
I A Iliya
Department of Human Anatomy, Ahmadu Bello University, Zaria
Nigeria
Login to access the Email id


DOI: 10.4103/2384-5147.176305

Rights and Permissions

Background: Diabetes mellitus is now one of the largest emerging pandemics of our time. Of the different types of diabetes mellitus, type 2 accounts for 90% of diabetic cases in humans worldwide. Insulin resistance followed by abnormal secretion of insulin from the pancreatic β-cells underlies the symptomatology of type 2 diabetes mellitus. Most investigations have assessed the hypoglycaemic potentials of s-allyl-cysteine and mangiferin by focusing on the biochemical and or pathophysiological changes. The histological and immunohistochemical changes have on the other hand received less attention. Aim: To evaluate the anti-diabetic potentials of s-allyl-cysteine (SAC), mangiferin (MAN) and a composite mixture of both (COM) in equal volume ratio (1:1) in type 2 diabetic Wistar rat models. Objective: This was achieved by evaluating the secretion of insulin in the pancreatic islets of diabetic and non-diabetic rats using immunohistochemistry techniques. Methodology: Eighteen (18) apparently healthy male albino Wistar rats (Rattus norvegicus) were grouped into 6 groups designated as non-diabetic control (NDC), diabetic control (DC), SAC, MAN, COM and glibenclamide (GLC). Insulin resistance was induced by first feeding the rats with a high-fat diet for a period of 10 weeks followed by a low dose of streptozotocin injection to induce type 2 diabetes mellitus. Therapeutic interventions was by the administration of 50 mg/kg body weight of SAC solution, 40 mg/kg body weight of MAN solution, equal volume ratio of both (SAC:MAN) and 5 mg/kg body weight of GLC. Results: Therapeutic interventions with the bioactive compounds significantly improved the glucose tolerance ability by ameliorating the hyperglycaemic condition in the diabetic rats which was significant at P < 0.05. Similarly, immunohistochemistry evaluation of the islet β-cells showed an increase in insulin secretion suggesting an improvement in glycaemic control and an eventual commitment of glucose to glycolysis. Conclusion: The amelioration of the type 2 diabetic mellitus by the bioactive compound therapies was due to the bioactive-mediated anti-hyperglycaemic and insulin release potentials. These potentials were observed to be more pronounced in the SAC group, followed by MAN group, then GLC group and lastly by COM group.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1896    
    Printed37    
    Emailed0    
    PDF Downloaded168    
    Comments [Add]    

Recommend this journal